Catalog ID: 6875 UNIT SIZE: 5 mg

JC-1 [Mitochondrial Membrane Potential Probe] [5,5',6,6'-tetrachloro-1,1',3,3'-tetraethylbenzimidazoylcarbocyanine iodide]

MOL. FORMULA

C25H27Cl4IN4

MOL. WEIGHT

652.23

CAS NUMBER

3520-43-2

MDL NUMBER

MFCD00188475

Description

JC-1 is a membrane permeable, cationic carbocyanine dye widely used for determining mitochondrial membrane potential in flow cytometry and fluorescent microscopy. This dye can selectively enter the mitochondria where it reversibly changes color as membrane potentials increase. This property is due to the reversible formation of JC-1 aggregates upon membrane polarization that causes shifts in emitted light from 530 nm (Em. JC-1 monomeric form) to 590 nm (Em. of J-aggregate) when excited at 488 nm. Both colors can be detected using filters for FITC and PE/phycoerythrin/rhodamine, respectively. JC-1 is both qualitative, in regards to the shift from green to orange fluorescence emission, and quantitative, as measured by fluorescence intensity, in both filter sets. JC-1 can be used to indicate the initiation of apoptosis. These characteristics make JC-1 a sensitive marker for mitochondrial membrane potential.

Documents

Safety Data Sheet

SDS: SDS 6875.pdf

Certificate of Analysis

Lot Number: 50601
Lot Number: ST14494
Lot Number: 50921

Product Manuals

No Documents Available

Images

No Images Available

Product Information

Product Size	5 mg
Form	Solid
Storage conditions	Refrigerate temperature (Ref) Protect from moisture (D) Protect from light (L)
Shipping conditions	Room temperature

Storage

Storage Condition
Store 4 to 8^oC (Ref)	Protect from Moisture (D)	Protect from Light (L)

Synonyms

• 1H-Benzimidazolium, 5,6-dichloro-2-[3-(5,6-dichloro-1,3-diethyl-1,3-dihydro- 2H-benzimidazol-2-ylidene)-1-propenyl]-1,3-diethyl-, iodide, (E)-

• CBIC2(3)

Citations And References

• Takatani T, Takahashi K, Uozumi Y, Shikata E, Yamamoto Y, Ito T, Matsuda T, Schaffer SW, Fujio Y, Azuma J. Am J Physiol Cell Physiol 287, C949-53 (2004)

• Sperelakis N, Daniel EE. Am J Physiol Gastrointest Liver Physiol 286, G234-43 (2004)

• L.B. Chen and S.T. Smiley, W.T. Mason, ed. Fluorescent and Luminescent Probes for Biological Activity, 124, (1993)

• S.T. Smiley et al. Proc. Natl. Acad. Sci. USA 88, 3671, (1991). 1; L.B. Chen and S.T. Smiley, W.T. Mason, ed. Fluorescent and Luminescent Probes for Biological Activity, 124, (1993)

Regulatory Compliance

For Research Use Only. Not for any animal or human therapeutic or diagnostic use No Animal source
Regulatory Compliance
6875	JC-1 (5,5',6,6'-Tetrachloro-1,1',3,3'-tetraethylbenzimidazolyl carbocyanine iodide) [CBIC2(3)]
OSHA Hazard		No Known OSHA Hazards
HMIS Classification		Health: 0	Flammability: 0	Physical Hazard: 0
NFPA Rating		Health: 0	Fire: 0		Reactivity: 0
TSCA Status		Not On TSCA Inventory