Catalog ID: 6063 UNIT SIZE: 25 mg

mBCl [Monochlorobimane]

MOL. FORMULA

C10H11ClN2O2

MOL. WEIGHT

226.66

CAS NUMBER

76421-73-3

MDL NUMBER

MFCD00077379

Description

Monochlorobimane is a low molecular weight, non-ionic, fluorescent probe for labeling thiols. This cell-permeant probe is essentially non-fluorescent until it reacts with a thiol group. The chloromethyl group is relatively selective for reaction with thiols at physiological pH but may also react with carboxylates at pH above 7. Monochlorobimane is useful to quantify and detect the distribution of glutathione in living cells and tissue homogenates. This low molecular weight label can also be used to derivatize thiol-containing proteins prior to electrophoresis without disturbing the protein’s mobility in the separation. The conjugates of monochlorobimane are optimally excited near 394 nm and have a variable emission near 490 nm. Please see related products below.

Documents

Safety Data Sheet

SDS: SDS 6063.pdf

Certificate of Analysis

Lot Number: 51102

Product Manuals

No Documents Available

Images

No Images Available

Product Information

Product Size	25 mg
Form	Solid
Category	Fluorescent Probes and Related Product: Bimane Reactive Probes and Related Products: Thiol-Reactive (Haloacetamide/Halobenzyl and Maleimide)
Absorption (estimated)	Wavelength (λmax) 380 ± 3 nm
Fluorescence (estimated)	Wavelength (λmax) 490± 4 nm, in buffer pH 8, after reaction with thiol
Storage conditions	Freeze: Store at -5 to -30°C (F) Protect from moisture (D) Protect from light (L)
Shipping conditions	Room temperature

Synonyms

• 1H,7H-Pyrazolo(1,2-α)pyrazole-1,7-dione, 3-(chloromethyl)-2,5,6-trimethyl

• Chlorobimane

Citations And References

• Culturing at atmospheric oxygen levels impacts lymphocyte function." Atkuri KR, Herzenberg LA, Herzenberg LA. Proc Natl Acad Sci U S A 102, 3756-9 (2005)

• Low-molecular-mass thiol compounds from a free-living highly pathogenic amoeba, Naegleria fowleri." Ondarza RN, Iturbe A, Hernandez E, Hurtado G. Biotechnol Appl Biochem 37, 195-204 (2003)

Regulatory Compliance

For Research Use Only. Not for any animal or human therapeutic or diagnostic use No Animal source
Regulatory Compliance
6063	mBCl [Monochlorobimane]
OSHA Hazard		No Known OSHA Hazards
HMIS Classification				Health: 0	Flammability: 0		Physical Hazard: 0
NFPA Rating				Health: 0	Fire: 0			Reactivity: 0
TSCA Status				Not On TSCA Inventory
Transportation Information
DOT (US)			IMDG			IATA
Not Dangerous Good			Not Dangerous Good			Not Dangerous Good