Catalog ID: 6203 UNIT SIZE: 25 mg

Resorufin-β-D-galactopyranoside

MOL. FORMULA

C18H17NO8

MOL. WEIGHT

375.33

CAS NUMBER

95079-19-9

MDL NUMBER

MFCD00064055

Description

Resorufin β-D-galactopyranoside is a fluorogenic substrate for β-galactosidase. β-galactosidase is a commonly used reporter to monitor for gene expression in molecular biology. Resorufin β-D-galactopyranoside is essentially non-fluorescent until β-galactosidase enzymatic activity releases the fluorescent dye, resorufin. Unlike fluorescein-based substrates, resorufin β-D-galactopyranoside releases the orange-fluorescent resorufin after a single enzymatic hydrolysis event with fast enzyme kinetics, making this substrate useful for flow cytometry, ELISA, and high throughput screening applications. Resorufin β-D-galactopyranoside may be used to detect β-galactosidase enzymatic activity in live cells after permeabilization or may be used to detect β-galactosidase enzymatic activity in cellular extracts. Enzymatic activity is detected by fluorescence with an excitation near 571 nm and fluorescence emission near 585 nm. Please see other related products at the bottom of this page.

Documents

Safety Data Sheet

SDS: MSDS 6203.pdf

Certificate of Analysis

Lot Number: 11399
Lot Number: 52033 24Nov2025

Product Manuals

No Documents Available

Images

No Images Available

Product Information

Product Size	25 mg
Form	Solid
Fluorescence (estimated)	Wavelength (λmax) 585 ± 4 nm for enzymatic hydrolysis product
Absorption (estimated)	Wavelength (λmax) 571 ± 3 nm for enzymatic hydrolysis product
Detection Method	Fluorescent enzymatic hydrolysis product
Storage conditions	Freeze: Store at -5 to -30°C (F) Protect from moisture (D) Protect from light (L)
Shipping conditions	Room temperature

Storage

Storage Condition
Store -5 to -30^oC (F)	Protect from Moisture (D)	Protect from Light (L)

Synonyms

• 3-phenoxazone 7-(β-D-galactopyranoside)

• 3H-Phenoxazin-3-one, 7-(β-D-galactopyranosyloxy)

Citations And References

• Crystallization of beta-galactosidase does not reduce the range of activity of individual molecules." Shoemaker GK, Juers DH, Coombs JM, Matthews BW, Craig DB. Biochemistry 42, 1707-10 (2003)

• Dispersive analysis of turnover rates of a CST reactor by flow-through microfluorometry under conditions of growth." Sernetz M, Willems H, Keiner K. Ann N Y Acad Sci 613, 333-337 (1990)

• A single-cell assay of beta-galactosidase activity in Saccharomyces cerevisiae." Wittrup KD, Bailey JE. Cytometry 9, 394-404 (1988)

Regulatory Compliance

For Research Use Only. Not for any animal or human therapeutic or diagnostic use No Animal source
Regulatory Compliance
6203	Resorufin-β-D-galactopyranoside
OSHA Hazard			No Known OSHA Hazards
HMIS Classification			Health: 1	Flammability: 0		Physical Hazard: 1
NFPA Rating			Health: 1	Fire: 0			Reactivity: 1
TSCA Status			Not On TSCA Inventory
Transportation Information
DOT (US)		IMDG			IATA
Not Dangerous Good		Not Dangerous Good			Not Dangerous Good