Catalog ID: 6277 UNIT SIZE: 25 mg

DiBAC₄(3)

Bis-(1,3-dibutylbarbituric acid) trimethine oxonol

MOL. FORMULA

C27H40N4O6

MOL. WEIGHT

516.64

CAS NUMBER

70363-83-6

MDL NUMBER

MFCD00467633

Description

DiBAC4(3) is a slow-response, anionic, lipophilic green-fluorescent dye that is commonly used for measurements of plasma membrane voltage potentials in live cells. The anionic oxonol dye enters depolarized cells where it binds to membranes and proteins and exhibits an increase in fluorescence intensity. Increasing depolarization in the cells results in increased influx of the anionic dye and a corresponding increase in fluorescence intensity. Hyperpolarization in the cells indicated by a loss of fluorescence intensity within the labeled cells. DiBAC4(3) is excited near 493 nm with an environmentally dependent fluorescence emission near 517 nm. Please see other related products at the bottom of this page.

Documents

Safety Data Sheet

SDS: SDS 6277.pdf

Certificate of Analysis

Lot Number: 50102
Lot Number: 50877
Lot Number: 51003
Lot Number: 51590
Lot Number: 51683

Product Manuals

No Documents Available

Images

No Images Available

Product Information

Product Size	25 mg
Form	Solid
Absorption (estimated)	Wavelength (λmax): 493 ± 3 nm
Fluorescence (estimated)	Emission Max: 517 ± 4 nm
Cell Permeability	Cell-permeant
Detection Method	Fluorescent
Storage conditions	Room temperature (RT) Protect from moisture (D) Protect from light (L)
Shipping conditions	Room temperature

Synonyms

• 2,4,6(1H,3H,5H)-Pyrimidinetrione, 1,3-dibutyl-5-(3-(1,3-dibutyl-1,2,3,4-tetrahydro-6-hydroxy- 2,4-dioxo-5-pyrimidinyl)-2-propenylidene)-

Citations And References

• Increased cell death in osteopontin-deficient cardiac fibroblasts occurs by a caspase-3-independent pathway." Zohar R, Zhu B, Liu P, Sodek J, McCulloch CA. Am J Physiol Heart Circ Physiol 287, H1730-9 (2004)

• Role of YidC in folding of polytopic membrane proteins." Nagamori S, Smirnova IN, Kaback HR. J Cell Biol 165, 53-62 (2004)

• Regulation of eosinophil membrane depolarization during NADPH oxidase activation." Bankers-Fulbright JL, Gleich GJ, Kephart GM, Kita H, O'Grady SM. J Cell Sci 116, 3221-6 (2003)

Regulatory Compliance

For Research Use Only. Not for any animal or human therapeutic or diagnostic use No Animal source
Regulatory Compliance
6277	DiBAC₄(3) [Bis-(1,3-dibutylbarbituric acid) trimethine oxonol]
OSHA Hazard			No Known OSHA Hazards
HMIS Classification			Health: 0	Flammability: 0		Physical Hazard: 0
NFPA Rating			Health: 0	Fire: 0			Reactivity: 0
TSCA Status			Not On TSCA Inventory
Transportation Information
DOT (US)		IMDG			IATA
Not Dangerous Good		Not Dangerous Good			Not Dangerous Good