B4F [Biotin-4-fluorescein]

Catalog ID: 6939 UNIT SIZE: 10 mg

B4F [Biotin-4-fluorescein]

1-4 ($133.00/Unit) 5-24 ($118.00/Unit) 25-49 ($105.00/Unit)


B4F [Biotin-4-fluorescein] is a convenient biotinylated fluorescent probe for accurate measurement of avidin and streptavidin and for unlabeled biotin in homogeneous fluorescence assays. It binds to avidin more readily than conventional fluorescein biotin.


Safety Data Sheet
SDS: SDS 6939.pdf
Certificate of Analysis
Lot Number: 50997
Product Manuals
No Documents Available


No Images Available

Product Information

Product Size 10 mg
Form Solid
Absorption (estimated) Wavelength (λmax): 494 ± 3 nm
Fluorescence (estimated) Emission Max: 524 ± 4 nm
Storage conditions Refrigerate temperature (Ref)
Protect from moisture (D)
Protect from light (L)
Shipping conditions Room temperature
  • • N-(Biotinylamidoethyl)-fluorescein-5-carboxamide  

    • N-(Fluorescein-5-carbamidoethyl)biotinamide

    • 2-(6-hydroxy-3-oxo-3H-xanthen-9-yl)-5-((2-(5-((3aS,4S,6aR)-2-oxohexahydro-1H-thieno[3,4-d]imidazol-4-yl)pentanamido)ethyl)carbamoyl)benzoic acid

Application of biotin-4-fluorescein in homogeneous fluorescence assays for avidin, streptavidin, and biotin or biotin derivatives. Ebner A, Marek M, Kaiser K, Kada G, Hahn CD, Lackner B, Gruber HJ, Journal, Methods Mol Biol (2008) 418:73-88

• Anomalous fluorescence enhancement of Cy3 and cy3.5 versus anomalous fluorescence loss of Cy5 and Cy7 upon covalent linking to IgG and noncovalent binding to avidin. Gruber HJ, Hahn CD, Kada G, Riener CK, Harms GS, Ahrer W, Dax TG, Knaus HG. Bioconjug Chem 11, 696-704 (2000)

• Rapid estimation of avidin and streptavidin by fluorescence quenching or fluorescence polarization." Kada G, Kaiser K, Falk H, Gruber HJ. Biochim Biophys Acta 1427, 44-48 (1999)



For Research Use Only. Not for any animal or human therapeutic

or diagnostic use

No Animal source

Regulatory Compliance


B4F [Biotin-4-fluorescein]

OSHA Hazard

No Known OSHA Hazards

HMIS Classification

Health: 0

Flammability: 0

Physical Hazard: 0

NFPA Rating

Health: 0

Fire:  0

Reactivity: 0

TSCA Status

Not On TSCA Inventory

Transportation Information




Not dangerous goods

Not dangerous goods

Not dangerous goods


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