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DAPI, dilactate  [4',6-Diamidino-2-phenylindole, dilactate]

Catalog ID: 7012 UNIT SIZE: 10 mg

DAPI, dilactate [4',6-Diamidino-2-phenylindole, dilactate]

C22H27N5O6
457.48
28718-91-4
MFCD00467366
1-4 ($79.00/Unit) 5-24 ($72.00/Unit) 25-49 ($67.00/Unit)

Description

DAPI dilactate is essentially the same as DAPI HCl (#6937) except in their counter ions. The dilactate counter ions make the dye more water soluble. DAPI shows blue fluorescence upon biniding DNA and also can be excited by UV. The blue fluorescent DAPI stain apparrantly associates with the minor groove of dsDNA, preferentially binding to AT clusters. DAPI is an excellent nuclear counterstain, showing a distinct banding pattern in chromosomes. It is also used for cell-cycle studies and mycoplasma detection. DAPI is a very sensitive dye that could be used to detect single nucleic acid molecules. DAPI has been shown to detect a single DNA molecule in solution, and to detect femtograms of DNA in single cells and chloroplasts.

Images

No Images Available

Documents

Safety Data Sheet
SDS: SDS 7012, 9372, 9373.pdf

Certificate of Analysis
Lot Number: 15176

Product Manuals
No Documents Available


 

Storage Condition

Store -5 to -30oC (F)

Protect from Moisture (D)

Protect from Light (L)

 

 

 

Product Specification Information

Catalog number: 7012     

Name:  DAPI, dilactate

4',6-Diamidino-2-phenylindole, dilactate

HPLC Purity: ≥ 90% at 254 nm

Absorption:

Wavelength Maximum λmax: 348 ± 3 nm in Methanol

Extinction Coefficient: 27,000 ± 3,500 cm-1 M-1

Fluorescence Emission Maximum λEm: 459 ± 4 nm in Methanol

NMR:  D2O

Appearance/Form: Yellow Solid

Solubility: DMSO, DMF, Methanol and Water

 

 

 

 

    4′,6-Diamidino-2-phenyindole, dilactate 

Inheritable histone H4 acetylation of somatic chromatins in cloned embryos." Wee G, Koo DB, Song BS, Kim JS, Kang MJ, Moon SJ, Kang YK, Lee KK, Han YM. J Biol Chem 281, 6048-57 (2006)
 
• Spreading of embryologically distinct urothelial cells is inhibited by SPARC." Hudson AE, Feng WC, Delostrinos CF, Carmean N, Bassuk JA. J Cell Physiol 202, 453-63 (2005)
 
Alternative lengthening of telomeres is characterized by high rates of telomeric exchange." Londono-Vallejo JA, Der-Sarkissian H, Cazes L, Bacchetti S, Reddel RR. Cancer Res 64, 2324-7 (2004)

 

 

For Research Use Only. Not for any animal or human therapeutic

or diagnostic use

No Animal source

Regulatory Compliance

7012

DAPI, dilactate  [4',6-Diamidino-2-phenylindole, dilactate]

OSHA Hazard

No Known OSHA Hazards

HMIS Classification

Health: 0

Flammability: 0

Physical Hazard: 0

NFPA Rating

Health: 0

Fire: 0

Reactivity: 0

TSCA Status

Not On TSCA Inventory

Transportation Information

DOT (US)

IMDG

IATA

Not dangerous goods

Not dangerous goods

Not dangerous goods

 

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